BIOLOGY 446
COURSE DESCRIPTION: A collection of laboratory exercises designed to provide practical exposure to some of the general principles and methodology of biochemistry. Techniques include photometry, polarimetry, electrophoresis, centrifugation, and various chromatographic techniques.
COURSE OBJECTIVES: Students will:
- Understand the principles of and be able to execute core techniques commonly used in modern biochemistry laboratories.
- Become proficient in buffer preparation, pipeting, basic spectrophotometry, quantification of proteins, carbohydrates and nucleic acids, and enzymatic assays.
- Become familiar with recombinant protein expression and purification using metal affinity chromatography, protein and DNA gel electrophoresis and immunoblotting.
- Learn how to keep a good-quality laboratory notebook and work independently in a research environment.
TOPICS COVERED:
- Pipeting
- Buffers and spectrophotometry
- Proteins assays
- Carbohydrate assays
- Measuring kinetics parameters of an inzyme
- Transformation of bacterial cells for protein expression
- Lyse pellets
- Protein purification
- Gel electrophoresis of protein fractions
- Immunblotting
